Purification from human plasma of a hexapeptide that potentiates the sulfation and mitogenic activities of insulin-like growth factors

Biochemical and Biophysical Research Communications
B DoussetF Belleville

Abstract

The human plasma contains small peptide molecules known as low molecular weight growth factors synergistically increasing certain biological actions of insulin-like growth factors. In the present work we isolated and characterized a hexapeptide with HWESAS as structure. This purified peptide was absolutely necessary for the sulfation activity of insulin-like growth factor-I on chick embryo pelvic cartilages and improved the mitogenic activity of both insulin-like growth factors. The effects of this hexapeptide were confirmed by using the homologous synthetic peptide, that exhibited similar biological effects. Other synthetic peptides with structure derived from hexapeptide were shown to be active: the pentapeptide HWESA appeared more potent than the tripeptide HWE, which is about 170 to 200 times less active than the hexapeptide. The sequence of hexapeptide HWESAS is identified in only one human protein that is C3f, a fragment of C3 complement.

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E R FroeschK D Gibson
Sep 1, 1988·The Journal of Clinical Endocrinology and Metabolism·M Binoux, P Hossenlopp
Jan 1, 1985·Annual Review of Physiology·E R FroeschJ Zapf
Jun 1, 1987·Analytical Biochemistry·M G Sargent
Feb 1, 1974·Acta Endocrinologica·J L van den Brande, M V Du Caju
Dec 11, 1974·Biochemical and Biophysical Research Communications·L FryklundH Sievertsson
Jun 16, 1980·Biochemical and Biophysical Research Communications·A Plet, Y Berwald-Netter
Mar 31, 1981·Biochemical and Biophysical Research Communications·M H HeulinP Nabet

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Citations

Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert DallmannSteven A Brown
Aug 17, 2018·Foot & Ankle International·Elizabeth M LeimerSamuel B Adams
Apr 28, 2016·PloS One·Elisabeth AltmaierGabi Kastenmüller
Mar 4, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·J CapiaumontB Dousset
Apr 26, 2019·Analytical Chemistry·Margarita SemisMarkus Kalkum

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