Purification, molecular cloning, and functional expression of dog liver microsomal acyl-CoA hydrolase: a member of the carboxylesterase multigene family

Archives of Biochemistry and Biophysics
M HosokawaK Chiba

Abstract

To clarify the reason for the high acyl-CoA hydrolase (ACH) activity found in dog liver microsomes, the ACH was purified to homogeneity using column chromatography. The purified enzyme, named ACH D1, exhibited a subunit molecular weight of 60 KDa. The amino terminal amino acid sequence showed a striking homology with rat liver carboxylesterase (CES) isozymes. ACH D1 possessed hydrolytic activities toward esters containing xenobiotics in addition to acyl-CoA thioesters, and these activities were inhibited by a specific inhibitor of CES or by CES RH1 antibodies. These findings suggest that this protein is a member of the CES multigene family. Since ACH D1 appears to be a protein belonging to the CES family, we cloned the cDNA from a dog liver lambdagt10 library with a CES-specific probe. The clone obtained, designated CES D1, possessed several motifs characterizing CES isozymes, and the deduced amino acid sequences were 100% identical with those of ACH D1 in the first 18 amino acid residues. When it was expressed in V79 cells, it showed high catalytic activities toward acyl-CoA thioesters. In addition, the characteristics of the expressed protein were identical with those of ACH D1 in many cases, suggesting that CES D1 encodes li...Continue Reading

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Citations

Feb 19, 2010·Expert Opinion on Drug Metabolism & Toxicology·Jeff L StaudingerCurtis D Klaassen
May 11, 2006·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S KuribayashiT Kamataki
Jul 17, 2015·Journal of Biochemical and Molecular Toxicology·Angela A BakerJason R Richardson
Oct 19, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masao MiyazakiAkemi Suzuki
Aug 22, 2006·Chemico-biological Interactions·Tetsuo Satoh, Masakiyo Hosokawa
Oct 19, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T B AdamsW J Waddell
Feb 29, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Masakiyo Hosokawa
Mar 16, 2005·The Journal of Biological Chemistry·Thomas LinkeEarl H Harrison
Apr 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tetsuo SatohBert N La Du
Jul 14, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Teruko ImaiKan Chiba
Jun 8, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Kenta MizoiMasakiyo Hosokawa

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