PMID: 8611186Feb 1, 1996Paper

Purification of 11 beta-hydroxysteroid dehydrogenase type 2 from human placenta utilizing a novel affinity labelling technique

The Biochemical Journal
R W BrownJ R Seckl

Abstract

11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) efficiently inactivates potent glucocorticoid hormones (cortisol and corticosterone), leaving aldosterone unmetabolized. Abundant 11 beta-HSD2 activity in human placenta plays a central role in controlling fetal glucocorticoid exposure, which if excessive is harmful and may predispose to low birth weight and hypertension in adulthood. Similar 11 beta-HSD2 activity in the distal nephron protects mineralocorticoid receptors from glucocorticoids and appears to be important in normal blood pressure control. We have purified human placental 11 beta-HSD2 16000-fold, to homogeneity, and determined over 100 residues of the internal amino acid sequence. Purification was assisted by a novel technique allowing highly specific (single spot on two-dimensional electrophoresis) photoaffinity labelling of active 11 beta-HSD2 in crude tissue extracts by its glucocorticoid substrates. This work reveals that 11 beta-HSD2 is a member of the short-chain alcohol dehydrogenase superfamily (apparent monomer M(r) approximately 40,000). It is a very basic (apparent pI = 9.1) intrinsic membrane protein, requiring as yet undefined membrane constituents for full stability. Affinity chromatography a...Continue Reading

Citations

Aug 25, 2016·Journal of Molecular Endocrinology·Gillian A GrayKaren E Chapman
Aug 1, 2013·Physiological Reviews·Karen ChapmanJonathan Seckl
Jun 24, 1998·British Journal of Obstetrics and Gynaecology·C KorebritsJ R Challis
Sep 19, 2018·Cellular and Molecular Life Sciences : CMLS·Ping ZhuKang Sun
May 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhangye XuBo Huang
Oct 27, 2007·Neurochemical Research·Karen E Chapman, Jonathan R Seckl
Jun 24, 2010·Physiological Genomics·Jonathan R ManningJohn J Mullins
May 21, 2021·The Tohoku Journal of Experimental Medicine·Xin GaoHironobu Sasano
Apr 23, 2003·Molecular and Cellular Endocrinology·Peter ArnoldAlex Odermatt
Aug 3, 2005·General and Comparative Endocrinology·Daniela Denari, Nora R Ceballos

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