PMID: 8589662Oct 1, 1995Paper

Purification of a Candida albicans germ tube specific antigen

FEMS Immunology and Medical Microbiology
A Marot-LeblondT Palmer

Abstract

In a previous work, Marot-Leblond et al. identified a Candida albicans germ tube-specific antigen by the use of a monoclonal antibody (mAb 3D9.3). In the present report, we used a two-step procedure to obtain a purified preparation of this antigen from a Zymolyase extract of Candida albicans germ tubes. The extract was first fractionated by gel filtration chromatography. The immunoreactive fractions were pooled, and the 3D9.3 antigen was further purified by hydrophobic interaction chromatography using a Phenyl-superose column. Analysis by SDS-PAGE, immunoblotting and Concanavalin A staining, revealed a single, polydisperse band ranging from 110 to 170 kDa. The antigen was purified 126-fold by protein content and 16.4-fold by carbohydrate content. Recovery of the antigen was 6.8% following the two-step purification.

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Citations

Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Mar 18, 2009·FEMS Immunology and Medical Microbiology·Bertrand BeucherRaymond Robert
Nov 21, 2007·Journal of Clinical Laboratory Analysis·Biljana DojnovZoran Vujcić
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W L ChaffinJ P Martínez
Feb 10, 1998·Clinical and Diagnostic Laboratory Immunology·R RobertJ M Senet
Jan 5, 2000·Journal of Clinical Microbiology·A Marot-LeblondR Robert

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