PMID: 7031658Nov 1, 1981Paper

Purification of a membrane-bound metalloendopeptidase from porcine kidney that degrades peptide hormones

Proceedings of the National Academy of Sciences of the United States of America
R MumfordM Zimmerman

Abstract

A porcine kidney microsomal metalloendopeptidase has been enriched 3900-fold. Gel filtration on a calibrated Toyo-Soda G-3000 SW column indicated an appropriate molecular weight for the endopeptidase of 88,000 +/- 2000. The purified enzyme is inhibited by a number of synthetic inhibitors of thermolysin. The endopeptidase hydrolyzes the succinyl (Suc)-containing fluorogenic peptide substrate Suc-Ala-Ala-Phe-(7-amino-4-methylcoumarin) at the Ala-Phe position with a Km of 2.9 X 10(-4) M. The endopeptidase also hydrolyzes a variety of peptides including corticotropin, substance P, angiotensin I and II, neurotensin, somatostatin, bradykinin, and the renin tetradecapeptide substrate. The endopeptidase hydrolyzes both [Leu]- and [Met]enkephalin at the Gly-Phe bond.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M ZimmermanW Troll
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D F VeberR Hirschmann
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A W StraussA W Alberts
Jan 1, 1980·Annals of the New York Academy of Sciences·A W StraussA W Alberts
Jan 30, 1981·Developmental Biology·R A MumfordM Zimmerman

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Citations

Feb 7, 1983·FEBS Letters·M Deschodt-Lanckman, A D Strosberg
Jan 1, 1985·The International Journal of Biochemistry·J S Bond, R J Beynon
Nov 1, 1983·Molecular and Cellular Endocrinology·E C Griffiths, J R McDermott
Feb 1, 1988·Molecular and Cellular Endocrinology·J A MolT J Visser
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J N Whitaker, M A Heinemann
Jan 1, 1997·Peptides·E Ottaviani, E Caselgrandi
Nov 3, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·K F StandevenP J Grant
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A ShippE L Reinherz
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E G McMahonM K Stern
Oct 17, 1983·European Journal of Biochemistry·S Blumberg, Z Tauber
Jul 1, 1985·European Journal of Biochemistry·R LauferZ Selinger
Dec 30, 1987·European Journal of Biochemistry·G M OlinsH A Zurcher-Neely
Oct 1, 1991·British Journal of Pharmacology·P D'Orléans-JusteA Claing
Jul 1, 1995·British Journal of Pharmacology·C HerbstT Simmet
Jun 1, 1987·The Journal of Clinical Investigation·N P Stimler-Gerard
Apr 1, 1989·The Journal of Clinical Investigation·A T LookS C Peiper
Nov 15, 1987·Archives of Biochemistry and Biophysics·N Marks, M J Berg
Jul 10, 1987·Biochimica Et Biophysica Acta·G M OlinsH A Zurcher-Neely
Feb 27, 1987·Biochemical and Biophysical Research Communications·B Malfroy, J Burnier
Feb 1, 1987·Biochemical Medicine and Metabolic Biology·Y WatanabeY Fujimoto
Dec 31, 1982·Biochemical and Biophysical Research Communications·R A MumfordA A Patchett
Jun 1, 1984·Archives of Biochemistry and Biophysics·R S RushL B Hersh
Sep 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M G SwainE A Jones

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