PMID: 9187271Jun 1, 1997Paper

Purification of a new dimeric protein from Cliona vastifica sponge, which specifically blocks a non-L-type calcium channel in mouse duodenal myocytes

Molecular Pharmacology
J L MorelMichel Hugues

Abstract

Marine sponges are synthesizing a wide variety of peptidic and organic molecules with biological activities. Multiple-step purification of Cliona vastifica extract led to a new dimeric peptide (mapacalcine; M(r) = 19,064) that is composed of two homologous chains, each containing nine cysteins. This protein has been found to selectively block a new calcium conductance characterized in mouse duodenal myocytes with an IC50 value of approximately 0.2 microM. The mapacalcine-sensitive current was a non-L-type calcium current activated from a holding potential of -80 mV that persisted during stimulation of the cell at high frequencies (0.1-0.2 Hz) within 5-10 min. Time constants of inactivation were similar for both L-type and non-L-type calcium currents. The non-L-type calcium current of duodenal myocytes was not blocked by the pharmacological agents specific for N-, L-, P-, or Q-type calcium channels. Mapacalcine was unable to block T-type calcium current in portal vein myocytes as well as voltage-dependent potassium currents and calcium-activated chloride currents in duodenal and portal vein cells. Mapacalcine did not affect caffeine-induced calcium responses, indicating that it did not interfere with intracellular calcium stores...Continue Reading

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Citations

Jun 15, 2000·The Journal of Biological Chemistry·P B Miarons, M Fresno
Feb 27, 2004·The Journal of Biological Chemistry·Jean-Luc MorelJean Mironneau
Jun 5, 2001·The Journal of Physiology·S D KohK M Sanders
Jul 12, 2013·PloS One·Hamid Moha Ou MaatiMichel Hugues
Apr 24, 2003·European Journal of Biochemistry·Dominique CrenesseMichel Hugues

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