Purification of a ribosome-inactivating protein with antioxidation and root developer potencies from Celosia plumosa

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Ashraf Gholizadeh

Abstract

Considering Celosia plumosa as a potent antiviral plant, the attempt was made to determine, purify and characterize its proteinaceous antiviral elements against tobacco mosaic virus hypersensitive response on Nicotiana glutinosa. By using 60% ammonium sulphate-precipitation, FPLC-based anion and cation-exchange chromatography in 10 and 50 mM NaCl, size-exclusion chromatography in 50 mM NaCl and SDS-PAGE 10%, a 25 kD antiviral protein with ribosome-inactivating/28S rRNase ability was purified from the leaves of C. plumosa at vegetative growth stage. The purified protein showed FRAP-based antioxidant activity in vitro and caused 1.7-fold and 1.4-fold increases in the growth rate of root system upon carborundum-based application on the root growth medium of N. glutinosa. The present work reports an antiviral protein with ribosome-inactivating, antioxidation and root developer potencies in C. plumosa as an edible or ornamental plant that may be useful in health and agricultural biotechnology in the future.

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Citations

Jan 28, 2021·Toxins·Lucía CitoresJosé M Ferreras

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Methods Mentioned

BETA
size-exclusion chromatography
electrophoresis
RIP
size exclusion chromatography

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