Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi

Journal of Applied Microbiology
Rosana Canuto GomesRosalie Reed Rodrigues Coelho

Abstract

The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological procedures placed it in the Streptomyces genus, but identification to species level could not be achieved. A protein with endochitinase activity was isolated and purified from the supernatant fluid by concentration, precipitation, hydrophobic interaction, gel filtration and adsorption procedures. The molecular size of the purified chitinase was estimated by gel filtration to be 70 kDa, and its pI was 6.1. The enzyme had temperature and pH optima of 40 degrees C and 8.0, respectively, and showed thermal (30-70 degrees C) and pH (4-9) stabilities. Antifungal activity of the selected strain was observed following in vitro experiments using growing cells, crude extract or the purified endochitinase, and by detecting growth inhibition of the tested phytopathogenic fungi. Strain Streptomyces RC 1071 could not be placed into any known species, suggesting a new taxon. The purified endochitinase presented similar molecular weight, optimum temperature an...Continue Reading

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Citations

Aug 24, 2004·Applied Microbiology and Biotechnology·Evi Andronopoulou, Constantinos E Vorgias
Jan 22, 2010·Applied Microbiology and Biotechnology·Shailesh R Waghmare, Jai S Ghosh
Feb 5, 2009·Applied Biochemistry and Biotechnology·Sanya Kudan, Rath Pichyangkura
Sep 27, 2002·Applied and Environmental Microbiology·A C MetcalfeE M H Wellington
Oct 16, 2004·The Journal of General and Applied Microbiology·Thongchai TaechowisanSaisamorn Lumyong
May 2, 2012·Pakistan Journal of Biological Sciences : PJBS·M RabeethGeetha Srikanth
Apr 17, 2014·Applied Microbiology and Biotechnology·Hiroki ShibasakiKoki Horikoshi
Mar 30, 2010·Journal of Bioscience and Bioengineering·Kazuaki SatoMakoto Shimosaka
Nov 20, 2008·Journal of Applied Microbiology·T D ZucchiI S de Melo
Aug 24, 2012·Journal of Basic Microbiology·Anand Nagpure, Rajinder K Gupta
Dec 9, 2008·International Journal of Systematic and Evolutionary Microbiology·Rodrigo Fonseca de SouzaRosana Canuto Gomes
Mar 14, 2013·BioMed Research International·Juliana Pacheco da RosaRosalie R R Coelho
Jul 19, 2013·Critical Reviews in Biotechnology·Anand NagpureRajinder K Gupta
Feb 28, 2007·Microbiological Research·Betania F QuirinoRicardo H Krüger
Jul 16, 2008·FEMS Microbiology Letters·Rosana Canuto GomesRosalie Reed Rodrigues Coelho
Jan 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yong ZhouXing Zhang
Dec 11, 2019·International Journal of Molecular Sciences·Elena Maria ColomboMatias Pasquali
Jun 8, 2018·Frontiers in Microbiology·Qaiser ShakeelLong Yang
Aug 23, 2020·Applied Biochemistry and Biotechnology·Gincy M MathewAshok Pandey

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