PMID: 9663692Jul 15, 1998Paper

Purification of a toxic factor from Arabian Gulf catfish epidermal secretions

Toxicon : Official Journal of the International Society on Toxinology
M ThomsonM Ali

Abstract

The Arabian Gulf catfish, Arius bilineatus (Valenciennes) secretes a proteinaceous epidermal secretion when threatened or injured. A toxic factor has been isolated and purified from the crude extract (crude skin toxin) of these secretions by a combination of gel filtration on Sephacryl S-300 and preparative discontinuous polyacrylamide gel electrophoresis. The purified skin toxin has a molecular weight of 39,000 Da and an isoelectric point (pI) of 5.45. Injection of the purified skin toxin into rabbits i.v. and determination of the LD50 indicated that the protein had been purified approximately 30 fold by these procedures. Injection of the purified skin toxin into rabbits caused agitation, convulsions and death within 5 min. Analysis of plasma levels of lactate dehydrogenase, glutamate-oxaloacetate transaminase and glutamate pyruvate transaminase in injected rabbits indicated that the skin toxin caused cardiac and liver damage to the animals.

References

Jun 1, 1975·Toxicon : Official Journal of the International Society on Toxinology·N PrimorE Zlotkin
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·M A AlnaqeebR S Criddle
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·A S GoldbergK D Barrow
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J M Al-HassanR S Criddle
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Al-LahhamR S Criddle
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J M Al-HassanR S Criddle
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·K ShiomiK Konno
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·J M Al-HassanC J Gubler
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Apr 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

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Citations

Dec 8, 2009·BMC Evolutionary Biology·Jeremy J Wright
Aug 16, 2011·Toxicon : Official Journal of the International Society on Toxinology·Saki TamuraKazuo Shiomi

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