PMID: 11318034Apr 25, 2001Paper

Purification of alpha-amylases using magnetic alginate beads

Applied Biochemistry and Biotechnology
S Teotia, M N Gupta

Abstract

Magnetic alginate beads were used to purify alpha-amylases from porcine pancreas, starchzyme, BAN 240L (a commercial purification from Bacillus subtilis), and wheat germ. The beads bound a significant level of alpha-amylase activity from porcine pancreas, BAN 240L, and wheat germ. In each case, the enzyme activity could be eluted by using 1.0 M maltose, a known competitive inhibitor of alpha-amylase. In the case of BAN 240L, 3.6-fold purification with 72% recovery of activity was observed. In the case of wheat germ enzyme, starting from the crude extract, 48-fold purification with 70% activity recovery was observed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis also indicated considerable purification in the latter case.

Citations

Sep 4, 2009·Applied Microbiology and Biotechnology·Kurutahalli S VishwanathaSridevi Annapurna Singh
Oct 29, 2003·Journal of Biotechnology·M SafaríkováI Safarík
Nov 30, 2004·Biomagnetic Research and Technology·Ivo Safarik, Mirka Safarikova
Jan 18, 2006·Archives of Insect Biochemistry and Physiology·Patrícia B PelegriniOctávio L Franco
Mar 16, 2006·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Sulakshana JainMunishwar N Gupta
Oct 21, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Munishwar N GuptaKusum Solanki

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