PMID: 7011404Mar 13, 1981Paper

Purification of an acidic alpha-D-mannosidase from Aspergillus saitoi and specific cleavage of 1,2-alpha-D-mannosidic linkage in yeast mannan

Biochimica Et Biophysica Acta
E IchishimaR Sumida-Tanaka

Abstract

An acidic alpha-D-mannosidase (alpha-D-mannoside mannohydrolase, EC 3.2.1.24) has been isolated from culture filtrate of Aspergillus saitoi. The extracellular alpha-mannosidase was homogeneous in polyacrylamide gel electrophoresis. The molecular weight of the enzyme was 51 000 and the isoelectric point pH 4.5. The purified enzyme has a pH optimum of 5.0, a Km of 0.45 mM with baker's yeast mannan and has no activity towards p-nitrophenyl-alpha-D-mannoside. The mode of action of the enzyme has been studied with baker's yeast mannan and saké yeast mannan. The enzyme cleaves specifically the 1,2-alpha-linked side chain, producing free mannose.

References

Dec 1, 1978·The Biochemical Journal·L J BurdittR D Jolly
Feb 1, 1974·The Biochemical Journal·N C PhillipsR D Jolly
Oct 16, 1980·Biochemical and Biophysical Research Communications·K YamashitaA Kobata
Feb 1, 1959·The Biochemical Journal·J CONCHIEG A LEVVY
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Nov 1, 1993·Current Microbiology·Y ChibaE Ichishima
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P K Gopal, C E Ballou
Aug 2, 2001·Glycobiology·A Kobata
Feb 13, 2014·Journal of Biochemistry·Jun-Ichi AikawaYukishige Ito
Jun 22, 2006·Journal of Biochemistry·Takeshi IshimizuSumihiro Hase
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Kukuruzinska, K Lennon
May 16, 1998·Biochemical and Biophysical Research Communications·E V EneyskayaK N Neustroev
Jul 19, 1995·Biochimica Et Biophysica Acta·S M GaikwadM I Khan
Feb 22, 2005·Carbohydrate Research·Vasileios I AthanasopoulosRobert A Rastall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.