PMID: 41584Dec 7, 1979

Purification of an alkaline nuclease from Physarum polycephalum

Biochimica Et Biophysica Acta
J H Waterborg, C M Kuyper

Abstract

An alkaline nuclease was purified from microplasmodia of Physarum polycephalum. The nuclease, active on denatured DNA and RNA and free of contamination by other nucleolytic activities, appeared to be a zinc-metallo protein. The enzyme was only active under conditions, where Zn2+ were retained in the enzyme. Loss of zinc occurred by the chelating action of EDTA, EGTA or ampholines, by acid of highly alkaline pH conditions or by high ionic strength. The addition of ZnCl2 to compensate losses, restored all activity, while all other divalent cations caused inhibition. The nuclease, with a molecular weight of 32 000, was stable at neutral pH at high temperatures with a half-life of 20 min at 80 degrees C. It was inhibited by any salt of buffer concentration above the level of zero ionic strength and showed a special sensitivity towards phosphate ions. The possible similarity of this enzyme to nuclease S1 from Aspergillus oryzae is pointed out.

Citations

Feb 15, 2003·FEMS Microbiology Reviews·Neelam A Desai, V Shankar
Jan 1, 1995·Critical Reviews in Microbiology·S U Gite, V Shankar
Feb 9, 1993·Biochemistry·M CzuprynB L Vallee

Related Concepts

Alkaline DNase
Edetic Acid, Calcium, Sodium Salt
Egtazic Acid Potassium Salt
Hydrogen-Ion Concentration
Study, Methodological
Osmolality
Physarum
Zinc

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