Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1

Oral Microbiology and Immunology
M BusarcevicL Topisirovic

Abstract

Lactobacillus salivarius BGHO1, a human oral isolate with antagonistic activity against growth of Streptococcus mutans, Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, and Salmonella enteritidis, probably produces more than one proteinaceous antimicrobial substance. The objective of this study was the purification of a bacteriocin, named LS1, produced by L. salivarius BGHO1. A simple and fast procedure for bacteriocin purification was developed, consisting of reverse-phase chromatography of the ammonium sulfate precipitate of cell-free culture supernatant by fast protein liquid chromatography and high-performance liquid chromatography, followed by tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with the subsequent extraction of bacteriocin from the gel. The supernatant of L. salivarius BGHO1 culture retained its antimicrobial activity after boiling in a water bath for 15 min. Its antimicrobial activity was also maintained even after treatment for 20 min at 121 degrees C in an autoclave. Bacteriocin LS1 was purified to homogeneity. The molecular mass of bacteriocin LS1 was estimated to be approximately 10 kDa, based on tricine SDS-PAGE. During purification...Continue Reading

References

Mar 1, 1988·Biochimie·T R Klaenhammer
Sep 1, 1993·International Journal of Food Microbiology·K I Garver, P M Muriana
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Oct 1, 1996·Antonie van Leeuwenhoek·I F NesH Holo
Feb 24, 1999·Critical Reviews in Food Science and Nutrition·A S NaiduR A Clemens
Sep 17, 1999·International Journal of Food Microbiology·T R Klaenhammer, M J Kullen
Feb 22, 2007·Oral Microbiology and Immunology·I StrahinicL Topisirovic

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Citations

Jan 11, 2011·Canadian Journal of Microbiology·Guillaume G Nicolas, Marc C Lavoie
Feb 23, 2012·The Journal of General and Applied Microbiology·Sirikhwan TinratMullika Traidej Chomnawang
May 10, 2013·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Wipapun RitthagolRawee Teanpaisan
Jun 29, 2012·International Journal of Antimicrobial Agents·Milos Busarcevic, Michèle Dalgalarrondo
Aug 2, 2011·Letters in Applied Microbiology·R TeanpaisanG Dahlén
Oct 15, 2013·Oral Diseases·C VuottoG Donelli
Sep 10, 2013·Food Microbiology·S MessaoudiX Dousset
Jun 29, 2010·FEMS Immunology and Medical Microbiology·Akio Tada, Nobuhiro Hanada
Jul 15, 2020·Journal of Periodontology·Purnima S Kumar, Kumar Subramanian
Jun 13, 2018·Journal of Food Science and Technology·Yogesh Kumar NegiS K Garg
Apr 25, 2015·Stomatologii︠a︡·Iu V ChervinetsA V Chervinets
Jan 5, 2021·Applied Microbiology and Biotechnology·Jelena LozoMilan Kojic
Jul 7, 2021·FEMS Microbiology Reviews·Abdelahhad BarbourMichael Glogauer

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