PMID: 4504350Jun 1, 1972Paper

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

Proceedings of the National Academy of Sciences of the United States of America
H Aviv, P Leder

Abstract

A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.

References

Jun 30, 1971·Nature: New Biology·L E MarounR M Nardone
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·H AvivP Leder
Feb 15, 1972·Biochemistry·G BrawermanS Y Lee
Sep 8, 1971·Nature: New Biology·H Burr, J B Lingrel
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D HousmanR Taber
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J E DarnellR J Tushinski
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S Y LeeG Brawerman
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M EdmondsH Nakazato

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Citations

Nov 26, 1999·Journal of Cellular Biochemistry·M S LeeC A Glackin
Jan 1, 1996·Molecular Reproduction and Development·L LiangG K Andrews
Aug 1, 1997·Molecular Reproduction and Development·S ErgünM S Davidoff
Apr 21, 1998·Molecular Reproduction and Development·I C GaemersD G De Rooij
Apr 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T Sato, R S Tuan
Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S B JakowlewA B Roberts
Nov 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S RebstockW Zimmermann
Jan 1, 1992·Developmental Genetics·F MeyerK Heckmann
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D T SchermerG J Strewler
Jun 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D J BenzB S Komm
Dec 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D T BaranJ Lian
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y Z WangS Christakos
Dec 1, 1989·Journal of Cellular Biochemistry·L L HsiehI B Weinstein
Aug 1, 1995·Journal of Cellular Biochemistry·S Senterre-LesenfantsM E Sobel
Oct 1, 1990·Journal of Cellular Physiology·F Chany-FournierC Chany
Aug 1, 1992·Journal of Cellular Physiology·G Chenevix-TrenchN K Hayward
Jul 1, 1990·Journal of Medical Virology·A LobbianiG Colucci
Aug 1, 1990·Journal of Neuroscience Research·J B WatsonJ G Sutcliffe
Oct 1, 1990·Journal of Neuroscience Research·T A RhynerB Pessac
Sep 1, 1991·Journal of Neuroscience Research·D R SoppetM B Willard
Apr 1, 1992·Journal of Neuroscience Research·Y Ichimura-OhshimaR Kuwano

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