Jun 1, 1972

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

Proceedings of the National Academy of Sciences of the United States of America
H Aviv, P Leder

Abstract

A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.

Mentioned in this Paper

Buffers
Centrifugation, Density Gradient
Chromatography
Polynucleotides
Reticulocytes
Tritium
Globin
Purification Aspects
Acrylamides
Irium

About this Paper

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