PMID: 6539128May 17, 1984Paper

Purification of biotin-binding protein from chicken egg yolk and comparison with avidin

Biochimica Et Biophysica Acta
C V Murthy, P R Adiga

Abstract

A simple alternative procedure for the purification in higher yields of the biotin-binding protein from the chicken egg yolk in a ligand-free form is described. The isolated protein was homogeneous by the criteria of polyacrylamide gel electrophoresis, gel filtration chromatography, immuno-double diffusion and immuno-electrophoresis. The protein had an Mr of 72 000 +/- 2000 and was a homotetramer of subunit Mr of 18 000 +/- 1000. It bound [14C]biotin in the molar ratio of 1:4 with an association constant (Ka) of 0.58 X 10(12) M-1. The yolk biotin-binding protein and avidin exhibited qualitatively similar spectral changes on interaction with biotin and p- hydroxyazobenzoic acid, but quantitatively these changes were more pronounced with avidin. Despite the lack of gross immunological cross-reactivity between the two biotin-binders, evidence based on immunological techniques for some degree of common conformational characteristics restricted to or around the ligand-binding sites of the two proteins was adduced. The mixed subunits of the two proteins failed to form hetero-oligomers on reconstitution.

References

Mar 1, 1979·The Biochemical Journal·K Muniyappa, P R Adiga
Dec 15, 1976·The Biochemical Journal·D R Fraser, J S Emtage
Jan 1, 1975·Advances in Protein Chemistry·N M Green
Aug 1, 1976·The Biochemical Journal·H B WhiteP H Sammelwitz
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Feb 1, 1974·Biological Reviews of the Cambridge Philosophical Society·R G Board, R Fuller
Mar 1, 1973·Analytical Biochemistry·J S Wolpert, M L Ernst-Fonberg
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Jan 1, 1970·Annual Review of Biochemistry·I M KlotzD W Darnall
Jan 1, 1970·Annual Review of Biochemistry·J Knappe
Mar 1, 1981·The Biochemical Journal·K Muniyappa, P R Adiga
May 1, 1962·The Biochemical Journal·J WILLIAMS
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L Bush, H B White
Mar 9, 2007·BMC Structural Biology·Vesa P HytönenTomi T Airenne
Mar 11, 1996·FEBS Letters·A V VieiraP M Vieira
Aug 15, 2009·Science·Laura L LacknerJodi Nunnari
May 8, 2010·Journal of Drug Targeting·Gregory Russell-JonesJohn McEwan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.