PMID: 8941966Sep 1, 1996Paper

Purification of bovine beta 2-microglobulin from colostrum and its complete amino acid sequence

Veterinary Immunology and Immunopathology
F HoshiS Kawamura

Abstract

Bovine beta 2-microglobulin (beta 2-m) was purified from colostrum milk in two chromatographic steps: anion-exchange chromatography, and gel filtration. The amino acid sequence was determined to confirm that the purified protein was beta 2-m. A molecular weight of 11.8 kDa beta 2-m was estimated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bovine beta 2-m consists of 98 amino acid residues and contains one disulfide linkage connecting residues 25 and 79. The amino acid sequence determined by this study is different from a previously published sequence at three sites, but agrees with the amino acid sequence from cDNA. Thus, we can conclude that the amino acid sequence determined in this study is correct.

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Citations

Jun 24, 1999·The Journal of Veterinary Medical Science·Y NakajimaS Kawamura
Sep 19, 2000·The Journal of Veterinary Medical Science·F HoshiS Kawamura

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