Purification of capsular polysaccharide produced by Haemophilus influenzae type b through a simple, efficient and suitable method for scale-up.

Journal of Industrial Microbiology & Biotechnology
Mickie TakagiJoaquin Cabrera-Crespo

Abstract

Haemophilus influenzae type b, an encapsulated bacterium, causes meningitis in infants worldwide. The capsular polysaccharide conjugated to a carrier protein is effective in the prevention of such infections. The traditional purification process of polysaccharide from bacterial cultures for vaccine production is based on several selective precipitations with solvents such as: ethanol, phenol, and cationic detergents. The separations of solid and liquid phases are based on continuous centrifugation in explosion proof installations. The lipopolysaccharides are separated by ultracentrifugation. A simple and efficient method that can easily be scaled-up was developed for purification of polysaccharides. The ethanol precipitation was reduced to only two steps. The phenol treatment was substituted by ultrafiltration and enzymatic digestion. Lipopolysaccharide was removed by ultrafiltration together with addition of detergent and chelating agent.

References

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Citations

Dec 15, 2015·Biotechnology Advances·Lise BesnardPriyabrata Pattnaik
Jul 1, 2015·Biologicals : Journal of the International Association of Biological Standardization·Sandeep SharmaManoj Kumar Chhikara
Dec 3, 2014·Carbohydrate Polymers·Silvia Maria Ferreira AlbaniJoaquin Cabrera-Crespo
Dec 3, 2014·Carbohydrate Polymers·Felipe de Oliveira Cintra, Mickie Takagi
Feb 15, 2021·Biologicals : Journal of the International Association of Biological Standardization·Suresh Beri, Darshan Gandhi
Sep 14, 2021·Glycoconjugate Journal·Iaralice Medeiros de SouzaIvna Alana Freitas Brasileiro da Silveira

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