PMID: 3753440May 1, 1986Paper

Purification of cathepsin B by a new form of affinity chromatography

The Biochemical Journal
D H RichA J Barrett

Abstract

Human cathepsin B was purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulphide at pH 4.0. The product obtained in high yield by the single step from crude starting material was 80-100% active cathepsin B. The possibility that this new form of affinity chromatography may be of general usefulness in the purification of cysteine proteinases is discussed.

Citations

Oct 7, 1998·Protein Science : a Publication of the Protein Society·P M SteedA K Knap
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