PMID: 2115615May 10, 1990Paper

Purification of dopa decarboxylase from bovine striatum

Molecular and Cellular Biochemistry
Y L Siow, K Dakshinamurti

Abstract

Pyridoxal phosphate-dependent DOPA decarboxylase has been purified from bovine striatum to a specific activity of 1.6 U/mg protein. After ammonium sulfate precipitation (30-60%) it was purified by DEAE-Sephacel, Sephacryl S-200, and TSK Phenyl 5 PW chromatography. The purified enzyme showed a single silver straining band with polyacrylamide gel electrophoresis under both denaturing and non-denaturing conditions. The bovine striatal DOPA decarboxylase is a dimer (subunit Mr = 56,000 by SDS-PAGE) with a native Mr of 106,000 as judged by chromatography on Sephacryl S-200 and by sedimentation analysis. Similar to the DOPA decarboxylase purified from non-CNS tissues, the bovine striatal enzyme requires free sulfhydryl groups for activity, is strongly inhibited by heavy metal ions, and can decarboxylate 5-hydroxytryptophan as well. It should be noted, however, that the final enzyme preparation is enriched in DOPA decarboxylase activity. The distribution of the DOPA decarboxylase and 5-HTP decarboxylase activities also varies among several bovine brain regions. In addition, heat treatment of the enzyme preparation inactivated the two decarboxylation activities at different rates.

References

Oct 12, 1978·Biochimica Et Biophysica Acta·K Srinivasan, J Awapara
Jan 2, 1979·European Journal of Biochemistry·C B VoltattorniC Turano
Nov 23, 1976·Experimental Brain Research·K DakshinamurtiV Havlicek
Nov 16, 1987·European Journal of Biochemistry·P DominiciC B Voltattorni
Jan 1, 1985·Experimental Brain Research·Y L Siow, K Dakshinamurti
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G ChristensonS Udenfriend
Nov 1, 1970·Archives of Biochemistry and Biophysics·J G ChristensonS Udenfriend
Jul 1, 1972·Canadian Journal of Biochemistry·G A Lancaster, T L Sourkes
Nov 1, 1970·Journal of Neurochemistry·J C SchwartzC Rose
Jan 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·H M PerryB M Sahagian
Sep 1, 1962·Archives of Biochemistry and Biophysics·J AWAPARAC HANLY
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Jan 1, 1993·Progress in Biophysics and Molecular Biology·M C Boadle-Biber
Feb 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S Bossinakou, E G Fragoulis
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P BradyE F Wawrousek
Sep 24, 2005·Neurochemical Research·Alice-Georgia VassiliouEmmanuel G Fragoulis
Aug 8, 2019·Cells·Efseveia FrakolakiNiki Vassilaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
M BuggeP Herlin
Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
P BartelmessM Bugge
© 2021 Meta ULC. All rights reserved