Purification of equine infectious anemia virus antigen by affinity chromatography.

Journal of Clinical Microbiology
T Sugiura, H Nakajima

Abstract

Affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (EIA) virus. After crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with EIA virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated Sepharose 4B to which EIA-specific antibody had been conjugated. The antigen was effectively released from the column with 5M MgCl2 and proved to be highly purified. Passive hemagglutination tests on sera from EAI infections were carried out, using the purified antigen. Results indicated that the passive hemagglutination test with the antigen was a specific laboratory test with high sensitivity for EIA infection.

References

Jan 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S L Morrison, M E Koshland
Jun 1, 1973·The Journal of General Virology·A R NeurathA Lippin
Sep 1, 1973·Japanese Journal of Microbiology·S UedaJ Nakamura
Jan 1, 1973·Archiv für die gesamte Virusforschung·H NakajimaK Hirasawa
Jan 1, 1973·Archiv für die gesamte Virusforschung·W A MalmquistC S Becvar
Aug 1, 1974·The Tohoku Journal of Experimental Medicine·H ShiraishiN Ishida
Feb 1, 1973·The Journal of Infectious Diseases·W O Grabow, O W Prozesky
May 7, 1971·Biochemical and Biophysical Research Communications·T OlivecronaU Lindahl
Mar 1, 1971·Infection and Immunity·H Nakajima, C Ushimi

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Citations

Jan 1, 1979·Journal of Medical Virology·F BrownK H Fantes

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