PMID: 676Nov 1, 1975

Purification of folate binding factor in normal umbilical cord serum

Proceedings of the National Academy of Sciences of the United States of America
B A Kamen, J D Caston

Abstract

Human umbilical cord serum was found to contain both free folate and folate complexed to a high-molecular weight factor. The complexed folate was bound to a very high affinity binder and was present in concentrations equivalent to as much as 60 ng of 5-methyltetrahydrofolic acid per ml of serum. Acidification of the serum caused disassociation of the folate-binder complex. Released folates were separated from binder by Sephadex gel filtration, zonal centrifugation through sucrose gradients, or adsorption onto activated charcoal. The separated binding factor, either saturated or unsaturated with folate, had a molecular weight of about 40,000 on Sephadex G-200 chromatography. Binding of [3H]pteroylglutamic acid was rapid and, as in the original endogenous folate-binder complex, was essentially irreversible at neutral pH. The affinity and specificity of the binder were examined by competition experiments using [3H]pteroylglutamic acid and nonradioactive folate derivatives. Oxidized folates were bound in preference to reduced derivatives, but only three to four times more unlabeled 5-methyltetrahydrofolic acid than pteroylglutamic acid was required to produce an equal level of competition. The strong affinity for 5-methyltetrahydro...Continue Reading

References

Sep 9, 1974·Biochemical and Biophysical Research Communications·M Zamierowski, C Wagner
Jan 1, 1972·Acta Haematologica·T MarkkanenP Himanen
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Sep 1, 1973·FEBS Letters·J Selhub, N Grossowicz
Jan 1, 1971·Acta Haematologica·T Markkanen, O Peltola
Feb 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·S P Rothenberg
Jan 1, 1969·Biochimica Et Biophysica Acta·N S LichtensteinI D Goldman
Sep 1, 1961·The Journal of Clinical Investigation·D G JOHNSA S BURGEN

Citations

Jul 1, 1995·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R P Steegers-Theunissen
May 1, 1980·The Journal of Pediatrics·S GrossD Caston
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Selhub, I H Rosenberg
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B A Kamen, A Capdevila
Mar 1, 1979·British Journal of Haematology·F Fernandes-Costa, J Metz
Mar 1, 1979·Acta paediatrica Scandinavica·J Ek, E M Magnus
Oct 1, 1989·European Journal of Pediatrics·N FogedO Blaabjerg
Sep 1, 1995·Baillière's Clinical Haematology·B Christensen, D S Rosenblatt
Aug 1, 1985·Journal of the American Geriatrics Society·D L Marcus, M L Freedman
Jul 15, 1986·Biochemical Pharmacology·B A Kamen, J D Caston
Jun 1, 1980·Archives of Biochemistry and Biophysics·G B HendersonF M Huennekens
Oct 8, 2013·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Ebru CelikBulent Berker
May 27, 2017·Journal of Agricultural and Food Chemistry·Sho WatanabeMitsuyoshi Ueda
Oct 1, 1980·Scandinavian Journal of Clinical and Laboratory Investigation·J HolmJ Lyngbye
Aug 24, 1999·The Journal of Veterinary Medical Science·M NatsuhoriE Kokue
May 11, 2006·The American Journal of Clinical Nutrition·Tsunenobu Tamura, Mary Frances Picciano
May 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rui YangRichard Gorlick

Related Concepts

Carrier Proteins
Umbilical Cord Blood
Folate
Hydrogen-Ion Concentration
Tetrahydrofolates

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