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Purification of four pyruvate kinase isozymes of rats by affinity elution chromatography

Biochimica Et Biophysica Acta

Jun 9, 1978

Kuniaki HaradaTeruyoshi Tanaka

PMID: 208618

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Abstract

1. Purification of four isozymes of pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) L, M1, M2 and R was much improved to give good yields by affinity elution chromatography. The enzyme was eluted from a phosphocellulose column with 0.5 mM phosphoenolpyruvate. Types L,...read more

Mentioned in this Paper

R-Type Pyruvate Kinase
Amino Acid [EPC]
Kinase Activity
Pyruvate Measurement
Phosphoenolpyruvate
Alloenzymes
Isoenzymes
Polymorphism, Crystallization
Phosphocellulose
Metazoa
Paper Details
References
  • References14
  • Citations16
12
  • References14
  • Citations16
12

Purification of four pyruvate kinase isozymes of rats by affinity elution chromatography

Biochimica Et Biophysica Acta

Jun 9, 1978

Kuniaki HaradaTeruyoshi Tanaka

PMID: 208618

DOI:

Abstract

1. Purification of four isozymes of pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) L, M1, M2 and R was much improved to give good yields by affinity elution chromatography. The enzyme was eluted from a phosphocellulose column with 0.5 mM phosphoenolpyruvate. Types L,...read more

Mentioned in this Paper

R-Type Pyruvate Kinase
Amino Acid [EPC]
Kinase Activity
Pyruvate Measurement
Phosphoenolpyruvate

Related Papers

Paper Details
References
  • References14
  • Citations16
12
  • References14
  • Citations16
12

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