Purification of galectin-3 from ovine placenta: developmentally regulated expression and immunological relevance

Glycobiology
M M IglesiasC Wolfenstein-Todel

Abstract

Galectins, beta-galactoside-binding lectins, are extensively distributed in the animal kingdom and share some basic molecular properties. Galectin-3, a member of this family, is generally associated with differentiation, morphogenesis, and metastasis. In this study, galectin-3 was isolated from ovine placental cotyledons round the middle of the gestation period by lactose extraction followed by affinity chromatography on lactosyl-agarose, and separated from galectin-1 by size exclusion chromatography on a Superose 12 column. Under native conditions this lectin behaved as a monomer with an apparent molecular weight of approximately 29,000 and an isoelectric point of 9.0. The partial amino acid sequence of the peptides obtained by tryptic digestion of this protein followed by HPLC separation showed striking homology with other members of the galectin-3 subfamily. Furthermore, ovine placental galectin-3 exhibited specific mitogenic activity toward rat spleen mononuclear cells. Besides, this protein strongly reacted with a rabbit antiserum raised against a chicken galectin. Results obtained by Western blot analysis showed that its expression was greatly decreased in term placenta with respect to the middle of the gestation period, ...Continue Reading

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Citations

May 10, 2007·Journal of Biomolecular NMR·Lianmei FengJames H Prestegard
May 22, 2003·Biochimica Et Biophysica Acta·María Mercedes IglesiasCarlota Wolfenstein-Todel
Nov 22, 2015·American Journal of Reproductive Immunology : AJRI·Melanie L ConradSandra M Blois
Feb 15, 2013·American Journal of Reproductive Immunology : AJRI·Ada G Blidner, Gabriel A Rabinovich
Jan 28, 2015·Journal of Reproductive Immunology·Sandra M BloisGabriela Barrientos
Aug 21, 2013·Journal of Reproductive Immunology·Sandra M Blois, Gabriela Barrientos
Sep 7, 2014·Cellular and Molecular Life Sciences : CMLS·Sandra M BloisAnne Cathrine Staff
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G A RabinovichC E Sotomayor
Jun 14, 2006·Glycobiology·Verónica A BironCarlota Wolfenstein-Todel
Mar 22, 2003·Histochemistry and Cell Biology·Ralf DahmMichael Kasper

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