PMID: 9543344Jun 6, 1998Paper

Purification of guinea pig small intestinal peroxisomes and the subcellular localization of glucose-6-phosphate dehydrogenase

Molecular and Cellular Biochemistry
P S TappiaM J Connock

Abstract

A method for the isolation of highly purified peroxisomes from guinea pig small intestine was developed. This two-stage process involved a rate-dependent banding of a light-mitochondria lambda-fraction followed by a density-dependent banding of the catalase enriched fractions obtained from the first step, using a horizontal rotor. Furthermore, the subcellular localization of glucose-6-phosphate dehydrogenase (NADP+-dependent) activity in guinea pig small intestine was examined. Analysis of density-gradient fractions indicated that approximately 3-4% of the cellular NADP+-dependent glucose-6-phosphate dehydrogenase activity is associated with peroxisomal fractions and that it is localized to the matrix of peroxisomes. It is therefore suggested that a peroxisomal source of NADPH may be utilized by enzyme systems that use NADPH specifically as a reductant.

Citations

May 20, 2015·The Protein Journal·Mahmoud A IbrahimMohammed M Abdel-Monsef
Jan 23, 2015·Enzyme Research·Mahmoud A IbrahimMohamed M Abdel-Monsef
Jan 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Nuray N Ulusu, Berivan Tandogan
Mar 30, 2010·Biochimica Et Biophysica Acta·M IslingerM Schrader

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