Purification of heat shock protein-peptide complexes for use in vaccination against cancers and intracellular pathogens

Methods : a Companion to Methods in Enzymology
P K Srivastava

Abstract

This article describes the methods used for the purification of heat shock proteins gp96, hsp90, and hsp70 from murine and human tissues and cell lines. The heat shock proteins purified by the methods described are associated noncovalently with an array of cellular peptides. The use of heat shock protein-peptide complexes for eliciting prophylactic and therapeutic immunity to cancers and infectious diseases is discussed.

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Citations

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