PMID: 2493266Feb 23, 1989Paper

Purification of high and low molecular weight plasminogen activator inhibitor 1 from fibrosarcoma cell-line HT 1080 conditioned medium

Biochimica Et Biophysica Acta
T Lindahl, Björn Wiman

Abstract

Functionally active (high-Mr) and inactive (low-Mr) plasminogen activator inhibitor 1 (PAI) have been purified from fibrosarcoma cell-line HT 1080 conditioned medium, containing 1% fetal calf serum. The two forms were first purified by affinity chromatography on heparin-Sepharose and then separated from each other by gel filtration on Sephadex G-150. The final purification was achieved by affinity chromatography on insolubilized monoclonal antibodies towards human PAI. Alternatively, the low-Mr form was purified by chromatography on carboxymethyl-cellulose. Low-Mr PAI purified in this way, could be almost fully reactivated by treatment with guanidinium chloride. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting revealed that the low-Mr form contained nothing but PAI at an Mr of about 50,000. In addition to PAI, the high-Mr form contained a component, which was not antigenically related to PAI. This compound had a molecular weight of about 75,000 and its NH2-terminal amino acid sequence corresponded to that of human vitronectin. We conclude that the high-Mr form of PAI constitutes a complex between 50,000 Mr PAI and vitronectin from fetal calf serum.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T NyD J Loskutoff
Nov 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·G UrdénB Wiman
Jul 1, 1986·ASAIO Transactions·J ChapmanL Martis
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L A EricksonD J Loskutoff
Jan 27, 1983·Biochemical and Biophysical Research Communications·J J EmeisG Wijngaards
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J LoskutoffD Lawrence
Aug 1, 1983·Thrombosis Research·J ChmielewskaB Wiman
Nov 8, 2012·Anaesthesia·J B Liban

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Citations

Jan 15, 1997·European Journal of Biochemistry·Z Gechtman, S Shaltiel
Nov 12, 2014·TheScientificWorldJournal·Songul Yasar YildizMehmet Agirbasli
Jul 8, 2000·FEBS Letters·H MångsB Wiman
Sep 24, 2004·Thrombosis Research·Haiyao WangBjörn Wiman
Dec 1, 1998·Scandinavian Journal of Clinical and Laboratory Investigation·A Nordenhem, B Wiman
Sep 21, 1994·Biochimica Et Biophysica Acta·O Sigurdardottir, B Wiman

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