PMID: 6172049Aug 1, 1981Paper

Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB

Allergy
J B TollR G Andersson

Abstract

This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.

References

Jan 1, 1978·Journal of Immunological Methods·V GhetieJ Sjöquist
Jun 1, 1976·Scandinavian Journal of Immunology·H Wigzell
Jan 1, 1970·International Archives of Allergy and Applied Immunology·J J Pruzansky, R Patterson
Jan 1, 1971·Advances in Immunology·H Bennich, S G Johansson
Apr 1, 1953·Proceedings of the Society for Experimental Biology and Medicine·J E MOORE, G W JAMES

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Citations

Apr 3, 1986·Journal of Immunological Methods·A A MiroliM Spitz
Feb 24, 1989·Journal of Immunological Methods·M A Lett-BrownJ A Grant
Sep 1, 1988·The Journal of Allergy and Clinical Immunology·H J DegenhartH J Neijens
Jan 1, 1988·Scandinavian Journal of Immunology·F J van OverveldJ Kreukniet

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