PMID: 6980256Aug 1, 1982Paper

Purification of human interleukin 2 to apparent homogeneity and its molecular heterogeneity

The Journal of Experimental Medicine
K WelteM A Moore

Abstract

Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt's lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. The purified IL-2 showed a 10(6) U/mg protein sp act. IL-2 produced in the absence of Daudi cells had a mol wt of 26,000 as measured by gel filtration and an isoelectric point of 6.7. This IL-2 showed a 16,000 and 17,000 mol wt in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). IL-2, produced in the presence of Daudi cells (10(6)/ml), showed a mol wt of approximately 14,000, as measured by both gel filtration and SDS-PAGE, and an isoelectric point of 8.1. The purified IL-2 lacked detectable interferon (alpha and gamma), granulocyte-macrophage colony-stimulating factor, B cell growth factor, T cell-replacing factor, and thymocyte-differentiating activity and was free of any contaminating proteins as judged by silver staining in SDS-PAGE. All three molecular forms of IL-2 were biologically active at concentrations of 10(-11) - 10(-10) M, supporting the growth of human and murine...Continue Reading

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Citations

Jul 6, 1984·Journal of Immunological Methods·O Bruserud, T Moen
Feb 24, 1984·Journal of Immunological Methods·B J MillsC W Todd
May 25, 1984·Journal of Immunological Methods·A GodardJ P Soulillou
Oct 24, 1985·Journal of Immunological Methods·A J GearingR Thorpe
Apr 1, 1984·Molecular Immunology·S M BellP L Stern
Jan 1, 1987·Pharmacology & Therapeutics·V Bocci
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Mar 1, 1988·Veterinary Immunology and Immunopathology·S R Sambhara, E L Belden
Jan 1, 1985·International Journal of Immunopharmacology·V J Merluzzi, K Last-Barney
Jan 1, 1984·Critical Reviews in Oncology/hematology·R V Smalley, R K Oldham
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R J RobbV Chowdhry
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A S SternS Gillis
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K WelteM A Moore
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C CernettiA Granelli-Piperno
Sep 1, 1984·The Journal of Experimental Medicine·A Granelli-PipernoE Reich
Oct 1, 1984·The Journal of Experimental Medicine·G TrinchieriB Perussia
Nov 1, 1984·The Journal of Experimental Medicine·K WelteR Mertelsmann
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Nov 15, 2008·Journal of Pharmaceutical Sciences·Mohamed A Shaker, Husam M Younes
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Mar 17, 2015·PloS One·Raghav PandeyAllan R Albig
Jan 1, 1985·Journal of Immunopharmacology·A E ChangS A Rosenberg

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