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Purification of human red cell glucose 6-phosphate dehydrogenase by affinity chromatography

Journal of Chromatography

Nov 26, 1975

A Yoshida

Abstract

Human glucose 6-phosphate dehydrogenase associated with NADPH was efficiently bound with agarose-bound NADP, whereas the enzyme associated with NADP was poorly bound with agarose-bound NADP. After the elimination of haemoglobin from haemolyzate by treatment with DEAE-cellulose, the enzy...read more

Mentioned in this Paper

Buffers
G6PD
Chromatography, DEAE-Cellulose
Phosphate Measurement
Glucosephosphate Dehydrogenase
Ampholytes
Enzyme agent
DEAE-Cellulose
Hemoglobin Measurement
Purification Aspects

Purification of human red cell glucose 6-phosphate dehydrogenase by affinity chromatography

Journal of Chromatography

Nov 26, 1975

A Yoshida

PMID: 416

DOI: 10.1016/s0021-9673(01)91996-x

Abstract

Human glucose 6-phosphate dehydrogenase associated with NADPH was efficiently bound with agarose-bound NADP, whereas the enzyme associated with NADP was poorly bound with agarose-bound NADP. After the elimination of haemoglobin from haemolyzate by treatment with DEAE-cellulose, the enzy...read more

Mentioned in this Paper

Buffers
G6PD
Chromatography, DEAE-Cellulose
Phosphate Measurement
Glucosephosphate Dehydrogenase
Ampholytes
Enzyme agent
DEAE-Cellulose
Hemoglobin Measurement
Purification Aspects

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