Purification of hydroxyanthraquinones and cinnamic acid from the roots of Rheum officinale Baill.

Journal of Separation Science
Yanyan HuangRenmin Liu

Abstract

A chromatographic method for isolation and purification of chemical constituents from the well-known traditional Chinese drug Da-huang (roots of Rheum officinale Baill.) was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. A two-step separation procedure is employed. Sixty five percent methanol was used as the eluent for separation of cinnamic acid, rhein, physcion and emodin form Da-huang crude extract. The fraction containing aloe-emodin and chrysophanol was then separated by using 55% methanol containing 0.5% acetic acid as the eluent. As a result, cinnamic acid and five kinds of hydroxyanthraquinones including rhein, aloe-emodin, chrysophanol, physcion and emodin were obtained. The retention behavior of hydroxyanthraquinones on Superose 12 was also studied. The retention of hydroxyanthraquinones on Superose 12 is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of the hydroxyanthraquinones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.

References

May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chun Yi LiauSimon J T Mao
Dec 9, 2004·Journal of Chromatography. a·Xiangling HeJan-Christer Janson
Oct 20, 2006·Journal of Chromatography. a·Jun XuJan-Christer Janson

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Citations

Oct 12, 2013·Journal of Chromatographic Science·Minglan YuRenmin Liu
Mar 25, 2019·Pharmaceutics·Constantin MircioiuIon Mircioiu

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