Purification of immunoglobulin G

Current Protocols in Immunology
Sarah M Andrew, Julie A Titus

Abstract

IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.

Citations

Jul 18, 2008·Current Protocols in Molecular Biology·Wayne M Yokoyama
Sep 29, 2020·Frontiers in Nutrition·Baidya Nath P SahDavid C Dallas
Apr 3, 2016·Current Protocols in Immunology·Karen Laky, Ada M Kruisbeek
Apr 25, 2018·Frontiers in Immunology·Mohammad Ameen Al-AghbarSteve R Roffler
Jun 7, 2021·Parasites & Vectors·Victor S NjomDavid J Buttle

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