PMID: 3320962Dec 23, 1987Paper

Purification of intercalator-released p67, a polypeptide that interacts specifically with the c-fos serum response element

Nucleic Acids Research
H SchröterA Nordheim

Abstract

Incubation of intact nuclei in buffers containing the DNA intercalating drug chloroquine leads to release of proteins that interact with DNA. We demonstrate here that a protein which binds to a motif within the human c-fos promoter, identified as the serum response element (SRE), is quantitatively released from HeLa nuclei, whereas nuclear factor 1 (NF 1) is not. Purification of the SRE binding protein by affinity chromatography to greater than 95% homogeneity allowed us to identify it as a polypeptide of approximately 67,000 daltons. The DNA contacts made by p67, as identified by methylation interference experiments, are indistinguishable from those of the serum response factor described previously.

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Citations

Jan 1, 1994·Advances in Enzyme Regulation·P HerrlichH J Rahmsdorf
Jan 1, 1990·Pharmacology & Therapeutics·H J Rahmsdorf, P Herrlich
Aug 2, 2002·European Journal of Pharmacology·Sangeeta Chawla
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PrywesR G Roeder
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Z LeviK Ozato
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B ToledanoW J Leonard
Feb 11, 1989·Nucleic Acids Research·S Leung, N G Miyamoto
Dec 25, 1991·Nucleic Acids Research·S L DanilitionN G Miyamoto
Jan 1, 1990·Cancer Investigation·C S FreemanC L Marks
Sep 30, 1999·Microscopy Research and Technique·G E Hardingham, H Bading
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M Weber, S M Levitz
Jul 1, 1997·Molecular and Cellular Biology·B S NikolajczykR Sen
Mar 1, 1991·Molecular and Cellular Biology·L RunkelA Nordheim
Mar 1, 1996·Molecular and Cellular Biology·H GilleP E Shaw
Jun 1, 1990·Genes & Development·J R ManakR Prywes

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