Purification of membrane attachment and inhibitory subunits of the proton translocating adenosine triphosphatase from Escherichia coli

Biochemistry
J B Smith, P C Sternweis

Abstract

The portion of Escherichia coli adenosine triphosphatase (ATPase) which is peripheral to the membrane (ECFl) is composed of five separate polypeptides referred to as alpha, beta, gamma, delta, and epsilon. Treating purified ECFl with pyridine precipitated the three larger polypeptides (alpha, beta, and gamma), but the two smaller ones (delta and epsilon), which represent only about 10% of ECFl, remained in solution. After removing the pyridine, both delta and epsilon were active and both were obtained in essentially pure form after chromatography on a single molecular-seive column. epsilon strongly inhibited the ATPase activity of ECFl, indicating that epsilon has a regulatory role in the enzyme. epsilon inhibited ECFl missing delta, indicating that delta is not required for inhibition by epsilon. However, enzyme containing just the alpha and beta subunits, which was prepared by treating ECFl with a protease, was fully active hydrolytically but not at all sensitive to inhibition by epsilon. This result suggests that the gamma polypeptide is required for the inhibition of the ATPase by epsilon. delta restored the capacity of ECFl missing delta to recombine with ECFl-depleted membrane vesicles. The ECFl, which became attached to ...Continue Reading

References

Jan 1, 1975·Journal of Supramolecular Structure·J B SmithLeon A Heppel
Apr 5, 1976·Biochemical and Biophysical Research Communications·A AbramsD H Morris
Feb 3, 1975·Biochemical and Biophysical Research Communications·J B Smith, P C Sternweis
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M FutaiLeon A Heppel
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·N NelsonD L Gutnick

Citations

Aug 1, 1979·Journal of Bioenergetics and Biomembranes·Y KagawaM Yoshida
Aug 1, 1988·Journal of Bioenergetics and Biomembranes·D C Gautheron, C Godinot
Feb 1, 1990·Journal of Bioenergetics and Biomembranes·T HashimotoK Tagawa
May 13, 2006·Journal of Bioenergetics and Biomembranes·Ken Yokoyama, Hiromi Imamura
Jan 1, 1987·The International Journal of Biochemistry·M A Rimpiläinen
Jul 3, 1979·Biochimica Et Biophysica Acta·B A Baird, G G Hammes
Sep 6, 1984·Biochimica Et Biophysica Acta·J E WalkerN J Gay
Aug 1, 1981·Journal of Inorganic Biochemistry·B M AlsaadiR C Woodworth
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R P GunsalusR D Simoni
Jan 5, 2011·The Journal of Biological Chemistry·Yusung KimT Hisabori
Apr 29, 2011·Journal of Biochemistry·Daichi OkunoH Noji
May 1, 1980·European Journal of Biochemistry·P D Bragg, C Hou
Dec 1, 1986·European Journal of Biochemistry·R G Tozer, S D Dunn
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sangjin Hong, P L Pedersen
Sep 27, 1991·Biochimica Et Biophysica Acta·J Mendel-Hartvig, R A Capaldi
Apr 16, 1985·Biochemical and Biophysical Research Communications·J P Aris, R D Simoni
Sep 1, 1987·Archives of Biochemistry and Biophysics·M Montero-Lomelí, G Dreyfus
Feb 15, 1984·Archives of Biochemistry and Biophysics·G Dreyfus, M Satre
Feb 15, 1984·Archives of Biochemistry and Biophysics·H Stan-Lotter, P D Bragg
Oct 1, 1979·Archives of Biochemistry and Biophysics·P P Laget, J B Smith
Oct 1, 1986·Archives of Biochemistry and Biophysics·K Schwerzmann, P L Pedersen
Sep 1, 1981·Archives of Biochemistry and Biophysics·S D Dunn, Leon A Heppel
Apr 28, 2004·Biochemical and Biophysical Research Communications·Hiroki KonnoT Hisabori
May 30, 1978·Biochemical and Biophysical Research Communications·S D Dunn
Apr 4, 2006·Biochimica Et Biophysica Acta·Mikhail A GalkinSteven B Vik
May 17, 2006·Biochimica Et Biophysica Acta·Boris A FenioukMasasuke Yoshida
Feb 22, 1990·Biochimica Et Biophysica Acta·S Engelbrecht, W Junge
Nov 24, 1999·The Journal of Biological Chemistry·Y Kato-YamadaM Yoshida
Jul 25, 1998·The Journal of Biological Chemistry·Y Kato-YamadaM Yoshida
Sep 17, 2004·The Journal of Biological Chemistry·Leon A Heppel
Nov 21, 1998·The Journal of Biological Chemistry·V V BulyginR L Cross
Feb 14, 2004·The Journal of Biological Chemistry·Hiromi ImamuraKen Yokoyama
Apr 2, 2011·The Journal of Biological Chemistry·Toshiharu SuzukiMasasuke Yoshida
Sep 30, 2018·Journal of Bioenergetics and Biomembranes·Francisco Mendoza-HoffmannJosé J García-Trejo
Jun 26, 1999·The Journal of Biological Chemistry·J WeberA E Senior

Related Concepts

DNA-dependent ATPase
Energy Metabolism
Alkalescens-Dispar Group
Cell Surface Proteins
Polypeptides
Protons
Structure-Activity Relationship

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.