PMID: 9433913Jan 20, 1998Paper

Purification of mitochondrial thioredoxin reductase and its involvement in the redox regulation of membrane permeability

Free Radical Biology & Medicine
Maria Pia RigobelloAlberto Bindoli

Abstract

The isolation to purity of a rat liver mitochondrial thioredoxin reductase is reported. The mitochondrial enzyme shows a chromatographic behavior different from that of the cytosolic enzyme. The purified enzyme, after sodium dodecylsulfate-polyacrylamide gel electrophoresis, yields a single band with a molecular weight of approximately 54 kDa. The apparent Km for E. coli thioredoxin is about 13 microM, while the apparent Km for 5,5'-dithiobis (2-nitrobenzoic acid) is 530 microM, values comparable to those reported for the cytosolic enzyme. Mitochondrial thioredoxin reductase, in addition to its natural substrate thioredoxin, is also able to reduce chemically unrelated compounds such as 5,5 '-dithiobis (2-nitrobenzoic acid), selenite, and alloxan; the enzyme is inhibited by classical inhibitors of the cytosolic enzyme such as 1-chloro-2,4-dinitrobenzene and 13-cis-retinoic acid. A strong inhibitory action is also elicited by Mn2+ and Zn2+ ions. Thiol status appears critically involved in the control of membrane permeability and, therefore, a thiol/disulfide transition involving reduced pyridine nucleotides, matrix soluble thiols, and inner membrane thiols appears to play a fundamental role. The potential role of thioredoxin/thio...Continue Reading

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Citations

May 23, 2001·Archives of Biochemistry and Biophysics·X LiJ M May
May 27, 1999·Journal of Biomedical Science·V N Gladyshev, D L Hatfield
Nov 4, 2010·The Journal of Microbiology·Hee-Jeong Seo, Young Nam Lee
Dec 1, 2001·Free Radical Biology & Medicine·J Nordberg, E S Arnér
Apr 3, 2001·Free Radical Biology & Medicine·M P RigobelloA Bindoli
Aug 7, 2002·British Journal of Pharmacology·Mark J McKeage
Aug 7, 2002·British Journal of Pharmacology·Maria Pia RigobelloAlberto Bindoli
May 23, 2001·The Biochemical Journal·M P RigobelloA Bindoli
Apr 24, 1999·European Journal of Biochemistry·A Miranda-VizueteG Spyrou
Sep 30, 2000·European Journal of Biochemistry·E S Arnér, A Holmgren
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Nov 9, 2011·Free Radical Biology & Medicine·Wenqing CaiJianguo Fang
Nov 25, 2003·The Journal of Investigative Dermatology·Isabella SaviniLuciana Avigliano
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Aug 23, 2005·Archives of Biochemistry and Biophysics·Maria Pia RigobelloAlberto Bindoli
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Jul 24, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Benoît BertrandEwen Bodio

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