PMID: 5257125Jun 1, 1969Paper

Purification of mitogenic proteins derived from Phaseolus vulgaris: isolation of potent and weak phytohemagglutinins possessing mitogenic activity

Proceedings of the National Academy of Sciences of the United States of America
L W AllenS Yachnin

Abstract

Commercially obtained phytohemagglutinin (PHAP) derived from Phaseolus vulgaris contains 17 different protein bands when analyzed by acrylamide gel electrophoresis. When it is subjected to CM-Sephadex chromatography followed by molecular sieving on Sephadex G150, several species of potent mitogenic proteins, which differ greatly in their hemagglutinating capacity, are obtained. A low hemagglutinating mitogen (L-PHAP), homogeneous by several different criteria, is the most potent mitogen isolated, and also possesses potent leukoagglutinating activity. It is a glycoprotein with a molecular weight of 115,000, containing glucosamine, mannose, xylose, and fucose or arabinose. Also isolated is a mixture of at least two closely related proteins possessing high hemagglutinating capacity, with hemagglutination titers 250 times more potent than L-PHAP. This material is a slightly less potent mitogen than L-PHAP and also possesses leukoagglutinating capacity, although of a lower order of magnitude. Its amino acid and carbohydrate composition are similar to L-PHAP, but it contains approximately twice as much carbohydrate and is slightly larger as determined by molecular sieving.

References

Aug 1, 1968·Transplantation·J S ThompsonH P Russe

❮ Previous
Next ❯

Citations

Jan 1, 1981·The International Journal of Biochemistry·R L FelstedR M Dale
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H HamazakiK Konishi
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R L FelstedN R Bachur
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M Harms-RingdahlL Ehrenberg
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J B MillerS Yachnin
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E P LesterS Yachnin
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Yachnin
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R A LarsonS Yachnin
Nov 1, 1994·Preparative Biochemistry·E ZentenoH Debray
Oct 1, 1973·The Journal of Experimental Medicine·J B MillerS Yachnin
Dec 10, 2002·Cancer Biotherapy & Radiopharmaceuticals·Bruce M Wimer, Paul L Mann
May 1, 1977·Plant Physiology·R de A Moreira, J C Perrone
Dec 1, 1975·British Journal of Haematology·R BraylanS Yachnin
Nov 1, 1978·Cell and Tissue Kinetics·C J Smith, J C Allen
May 15, 1984·European Journal of Biochemistry·A VitaleM J Chrispeels
Sep 13, 1974·Biochimica Et Biophysica Acta·A Pusztai, W B Watt
Oct 3, 1993·Biochimica Et Biophysica Acta·K KamemuraT Takahashi
Dec 21, 1976·Biochimica Et Biophysica Acta·E Saltvedt
Aug 14, 1970·Biochimica Et Biophysica Acta·A Novogrodsky, E Katchalski
Jan 28, 1971·Biochimica Et Biophysica Acta·A Novogrodsky, E Katchalski
Apr 1, 1972·Life Sciences. Pt. 1: Physiology and Pharmacology·T H WeberG V Alm
Feb 1, 1972·Cellular Immunology·R A JohnsonC H Kirkpatrick
Dec 3, 1971·Biochemical and Biophysical Research Communications·Y AbeS I Ishii
Jan 1, 1982·Metabolic Bone Disease & Related Research·T M WangJ L Matthews
Sep 1, 1971·Biochemical and Biophysical Research Communications·D Allan, M J Crumpton
Oct 1, 1972·Archives of Biochemistry and Biophysics·Y H O, R A Conard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.