Purification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography.

Plant Physiology
E A Funkhouser, F Loewus


myo-Inositol 1-phosphate synthase (EC is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold in a single step, about 9000-fold over-all, to a specific activity of 0.078 units per milligram of protein. This is an order of magnitude greater than previous purifications of the plant enzyme.


Sep 1, 1974·Archives of Biochemistry and Biophysics·W F NaccaratoW W Wells
Mar 22, 1967·Biochimica Et Biophysica Acta·E Piña, E L Tatum
Oct 1, 1973·Biochemical and Biophysical Research Communications·S M ByunS Kirkwood
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P CuatrecasasC B Anfinsen
Jun 1, 1973·Plant Physiology·H B LiebC C Still

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Nov 25, 1997·Biochimica Et Biophysica Acta·A L MajumderS A Henry
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