Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography

Journal of Chromatography. a
F ChenYue Jiang

Abstract

High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%.

Citations

Feb 23, 2011·International Journal of Molecular Sciences·Chunhua ZhouJun Tao
Jun 6, 2014·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Krystyna Skalicka-Woźniak, Ian Garrard
Jul 20, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Jianyu ZhouJianjun Zhang
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Da-Zhi LiuXing-Zu Zhu
Dec 15, 2004·Phytochemical Analysis : PCA
Jun 6, 2020·Frontiers in Pharmacology·Yan-Song LuHong-Duo Chen

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