PMID: 9433457Jan 20, 1998Paper

Purification of Pf155/RESA antigen from supernatants of in vitro Plasmodium falciparum cultures by continuous elution electrophoresis

Journal of Immunological Methods
B Dubois, P Deloron

Abstract

The malaria parasite Plasmodium falciparum synthesizes a protein, Pf155/RESA, which associates with the membrane of newly invaded erythrocytes. Using spent supernatants from P. falciparum growing in culture as a source of soluble Pf155/RESA, we have developed a purification technique based on the concentration of these supernatants, followed by gel filtration and continuous elution electrophoresis. SDS-PAGE electrophoresis and immunoblots were used to establish the quality of the purification.

References

Aug 20, 1976·Science·W Trager, J B Jensen
Oct 1, 1990·Experimental Parasitology·M AikawaL H Miller
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·S JepsenN H Axelsen
Jul 1, 1983·The American Journal of Tropical Medicine and Hygiene·A A GabrielsenM T Boland

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