Purification of Phenylpropanoids from the Scaly Bulbs of Lilium Longiflorum by CPC and Determination of Their DPP-IV Inhibitory Potentials

ACS Omega
Bo-Ram KimJoo-Won Nam

Abstract

The scaly bulbs of Lilium longiflorum (Liliaceae) are used as a food ingredient and a traditional medicine in East Asia. A preliminary study revealed that treatment with 100 μg/mL of the ethyl acetate fraction of this plant material inhibited dipeptidyl peptidase IV (DPP-IV) to 58.99%. Phytochemical studies were conducted to identify the active ingredient, and five compounds, namely, 1 (2.9 mg, 75.8% purity at 320 nm), 2 (12.2 mg, 97.9% purity at 320 nm), 3 (3.1 mg, 66.5% purity at 320 nm), 4 (6.8 mg, 96.9% purity at 320 nm), and 5 (6.2 mg, 90.2% purity at 320 nm) were purified from 200 mg of the ethyl acetate fraction of L. longiflorum via centrifugal partition chromatography (CPC) with a two-phase solvent system composed of chloroform/methanol/isopropanol/water (5:2:2:4, v/v/v/v) in an ascending mode. Their structures were identified as 1-O-p-coumaroyl-2-O-β-glucopyranosylglycerol (regaloside D, 1), 3,6'-O-diferuloylsucrose (2), 1-O-p-coumaroyl-2-O-β-glucopyranosyl-3-O-acetylglycerol (regaloside B, 3), 1-O-p-coumaroylglycerol (4), and 4-O-acetyl-3,6'-O-diferuloylsucrose (5), respectively, by 1H and 13C NMR and MS analysis. Compounds 2 and 5 exhibited DPP-IV inhibitory activities with IC50 values of 46.19 and 63.26 μM, respect...Continue Reading

References

Mar 1, 1996·Phytochemistry·T SatouY Hatakeyama
Apr 13, 1999·Cell·S I Taylor
Jul 28, 2006·Carbohydrate Research·M Iqbal Choudhary Atta-ur-Rahman
Aug 5, 2010·Journal of Agricultural and Food Chemistry·John P MunafoThomas J Gianfagna
Mar 14, 2012·Diabetes Research and Clinical Practice·Hamendra Singh ParmarAnil Kumar
Sep 27, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Junfeng FanElvira Gonzalez de Mejia
Jun 3, 2014·Journal of Agricultural and Food Chemistry·Allyson M BowerElvira Gonzalez de Mejia
Nov 20, 2014·Natural Product Reports·John P Munafo, Thomas J Gianfagna
Dec 4, 2014·Bioorganic & Medicinal Chemistry Letters·Ki Hyun KimKang Ro Lee
Apr 24, 2015·Journal of Agricultural and Food Chemistry·John P Munafo, Thomas J Gianfagna
Jun 13, 2015·Future Medicinal Chemistry·Yuanyuan GaoYiming Li
Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Sep 24, 2015·Planta medica·Yang LiuGuido F Pauli
Feb 1, 2017·Journal of Separation Science·Małgorzata BojczukPaweł Hodurek
May 12, 2018·European Journal of Medicinal Chemistry·Nagaraju KerruParvesh Singh
Jun 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Poonam KalhotraTzayhri Gallardo-Velázquez
Aug 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo-Ram KimAh-Reum Han

❮ Previous
Next ❯

Citations

Sep 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bo-Ram KimAh-Reum Han

❮ Previous
Next ❯

Methods Mentioned

BETA
pharmacotherapy
NMR
Assay

Software Mentioned

GraphPad Prism
ChemStation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.