Purification of plasma vitamin D metabolites for radioimmunoassay

Clinica Chimica Acta; International Journal of Clinical Chemistry
G A TaylorA Holmes

Abstract

The ability of four solvent systems to extract tritiated 25-hydroxy vitamin D3, 24,25-dihydroxy vitamin D3, 25,26-dihydroxy vitamin D3 and 1 alpha, 25-dihydroxy vitamin D3 from plasma was compared. Diethyl ether gave the highest yield of metabolites and lowest yield of "lipid" materials, the dihydroxylated metabolites were more readily extracted than 25-hydroxy vitamin D3. Following extraction with ether the vitamin D metabolites could be separated, without prior purification, on a 6.2 mm X 250 mm Zorbax-Sil high pressure liquid chromatography (HPLC) column, a simplification of previous methods. Plasma 1 alpha, 25-dihydroxy vitamin D levels measured after purification by this method were not significantly different from those obtained by an established, more complex method.

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Citations

Oct 1, 1991·Calcified Tissue International·H A MorrisB E Nordin
May 1, 1990·Calcified Tissue International·H A MorrisB E Nordin
Sep 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·W B Brown, M Peacock
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Apr 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Allan G NeedB E Christopher Nordin
Jun 6, 2000·The American Journal of Clinical Nutrition·A G NeedB C Nordin

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