Purification of primordial germ cells from TNAPbeta-geo mouse embryos using FACS-gal

Developmental Biology
K AbeK i Yamamura

Abstract

Tissue nonspecific alkaline phosphatase (TNAP), the product of the Akp2 locus, is expressed in mouse primordial germ cells (PGC) for an extensive period during embryogenesis. Mice with the Akp2tm1Sor mutant allele of TNAP express lacZ (beta-galactosidase; beta-gal) under control of the Akp2 locus. PGCs were purified from Akp2tm1Sor embryos using fluorescence activated cell sorting of beta-gal expressing cells (FACS-gal). Analysis of the purified cells by alkaline phosphatase staining and immunocytochemistry with anti-c-kit antibody demonstrated that highly (98%) purified PGCs can be isolated using this method. This technique will facilitate experiments that require highly purified preparations of PGCs including cell culture and gene expression analyses.

Citations

Nov 17, 2001·Trends in Biotechnology·M S Ko
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T UedaH Sasaki
Jan 26, 2000·Development, Growth & Differentiation·T YoshimizuY Matsui
May 11, 2012·Stem Cells and Development·Emma BellNicholas Hole
Feb 10, 2006·Development, Growth & Differentiation·Shuji ShigenobuSatoru Kobayashi
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Jun 24, 2009·Genesis : the Journal of Genetics and Development·Shirley S Hammond, Angabin Matin
Sep 2, 2006·Stem Cells·Hidetoshi YamazakiShin-Ichi Hayashi
Jul 7, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Hilda Amalia Pasolli
Sep 5, 2008·Current Protocols in Cytometry·M Lorincz, M Roederer
Mar 7, 2003·Development·Ana Sofia CachaçoSólveig Thorsteinsdóttir
Jun 9, 2001·Developmental Biology·M E Pepling, A C Spradling
Aug 30, 2003·Science·Masayuki TsudaYumiko Saga
May 22, 2008·Reproductive Biomedicine Online·Deshira Saiti, Orly Lacham-Kaplan

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