Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli

Protein Expression and Purification
Eric J HebertC Nick Pace

Abstract

The genes for three small ribonucleases from different strains of Streptomyces aureofaciens have been cloned and expressed in Escherichia coli. The purification of these ribonucleases from the periplasmic space is described. The yields range from 10 to 50 mg of protein per liter of culture medium. The molar absorption coefficients, isoelectric pH values, and pH of optimum activity are reported.

Citations

Sep 19, 2002·Protein Science : a Publication of the Protein Society·Olga N IlinskayaAlexander A Makarov
May 23, 2001·Protein Science : a Publication of the Protein Society·Kevin L ShawC Nick Pace
Sep 24, 1999·Protein Science : a Publication of the Protein Society·Gerald R GrimsleyC Nick Pace
Jun 24, 1998·Journal of Molecular Biology·C Nick PaceGerald R Grimsley
May 26, 2004·Biochemical and Biophysical Research Communications·Alexander A MakarovRonald T Raines
Feb 17, 2005·Journal of Molecular Biology·Travis T WaldronKenneth P Murphy
Jul 17, 2001·Proteins·D LaurentsM Bruix
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Gennady I YakovlevAlexander A Makarov
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Jason P Schmittschmitt, J Martin Scholtz
Mar 5, 2014·Protein Science : a Publication of the Protein Society·C Nick PaceGerald R Grimsley
Jun 7, 2005·Protein Science : a Publication of the Protein Society·Jared M TrefethenDavid N Brems
Mar 4, 2010·Protein Science : a Publication of the Protein Society·C Nick PaceGerald R Grimsley
Mar 27, 2010·Protein Science : a Publication of the Protein Society·Hailong FuC Nick Pace
Jul 25, 2009·Proteins·Hailong FuC Nick Pace
Oct 5, 2015·World Journal of Microbiology & Biotechnology·E Esin Hameş, Tuğçe Demir
Sep 14, 2002·The Journal of Biological Chemistry·Jozef SevcikRonald T Raines
Jan 17, 2015·Oncoscience·Vladimir A MitkevichAlexander A Makarov
Jun 12, 2003·The Journal of Biological Chemistry·Kazufumi TakanoC Nick Pace

Related Concepts

Ribonuclease Sa2
Alkalescens-Dispar Group
Shuttle Vectors
Alloenzymes
Proteins, Recombinant DNA
Alkaline Ribonuclease
Streptomyces
Gene Expression
Homologous Sequences, Amino Acid

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