Purification of soluble enzymes from erythrocyte hemolysates by three phase partitioning

The International Journal of Biochemistry
M C PolL Visser

Abstract

1. The three phase partitioning method of protein fractionation was successfully applied to human erythrocyte hemolysates for the removal of hemoglobin and the concentration of soluble enzymes. 2. Human carbonic anhydrase I and II, catalase and superoxide dismutase were recovered free of hemoglobin and in good yield in the initial partitioning step, with a 60- to 80-fold enrichment of enzyme activities. 3. After further purification, carbonic anhydrases I and II were obtained at overall yields of 84 and 29%, respectively, crystallized catalase at 38% and superoxide dismutase at 52%.

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Citations

Dec 14, 2011·Advances in Urology·Ricardo Miyaoka, Sandro C Esteves
Jun 14, 2017·Critical Reviews in Food Science and Nutrition·Jing-Kun YanJian-Yong Wu
Jan 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shingo KurabuchiEdward W Gresik
May 25, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S KurabuchiE W Gresik
Jan 1, 1994·Microscopy Research and Technique·E W Gresik
Jun 10, 2009·Vector Borne and Zoonotic Diseases·Carezza Botto-MahanAldo Solari
Jan 7, 1998·Protein Expression and Purification·C Dennison, R Lovrien
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·W P Michalski
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·N Bergenhem

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