Purification of storage granule protein-23. A novel protein identified by phage display technology and interaction with type I plasminogen activator inhibitor.

The Journal of Biological Chemistry
I M LangR R Schleef

Abstract

Type 1 plasminogen activator inhibitor (PAI-1) is a key regulator of the fibrinolytic cascade that is stored in a rapidly releasable form within platelet alpha-granules. To identify proteins that may participate in the targeting or storage of this potent inhibitor, this report investigates the applicability of utilizing filamentous bacteriophages to display proteins expressed by cells containing a regulated secretory pathway and their enrichment based upon an interaction with PAI-1. For this purpose, RNA was extracted from AtT-20 cells (i.e. a classical model cell system for intracellular protein sorting), reverse transcribed, amplified using polymerase chain reaction primers containing internal restriction sites, and cloned into the phagemid pCOMB3H for expression as fusion constructs with the bacteriophage gene III protein. Escherichia coli was transformed with the phagemids and infected with VCSM13 helper phage, and the resulting AtT-20 cDNA-bacteriophage library was enriched by panning against solid- and solution-phase PAI-1. The enriched cDNA library was subcloned into a prokaryotic expression vector system that replaces the gene III protein with a decapeptide tag for immunologic quantitation. One novel cDNA clone (i.e. A-...Continue Reading

References

Jul 1, 1992·Trends in Biochemical Sciences·J K Scott
Aug 1, 1992·Current Opinion in Cell Biology·M J Rindler
Nov 20, 1992·Science·R A LernerC F Barbas
Aug 1, 1991·Current Opinion in Cell Biology·R B Kelly
Feb 1, 1991·Journal of Clinical Pathology·A J SimpsonB Bennett
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasS J Benkovic
May 1, 1989·The Journal of Clinical Investigation·R R SchleefL J Levitt
Jul 18, 1986·Cell·R RossD F Bowen-Pope
Nov 1, 1988·British Journal of Haematology·N A BoothI R MacGregor
Jan 1, 1987·Annual Review of Cell Biology·T L Burgess, R B Kelly
Jul 1, 1985·Journal of Clinical Pathology·N A BoothB Bennett
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T B BarrettE P Benditt
Oct 1, 1984·The Journal of Clinical Investigation·L A EricksonD J Loskutoff
Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·L M Fink, P L Hsu
Mar 11, 1995·Nucleic Acids Research·M HottigerU Hübscher
Apr 1, 1993·Current Opinion in Immunology·D P Lane, C W Stephen
Jan 1, 1994·Advances in Immunology·D R Burton, C F Barbas
Jan 1, 1994·Annual Review of Immunology·G WinterH R Hoogenboom
May 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·B A KonkleE M Mazur
Feb 2, 1996·The Journal of Biological Chemistry·I M Lang, R R Schleef

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Oct 16, 2002·Biochemical and Biophysical Research Communications·E Helen KempPhilip P Watson
Jun 20, 2006·Journal of Virological Methods·Diana Jia Miin OoiJennifer Ann Harikrishna

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