Purification of the eukaryotic 20S proteasome

Current Protocols in Protein Science
Sherwin Wilk, W E Chen

Abstract

The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As described in this unit, the constitutive form of the 20S proteasome can be conveniently purified as a stable and homogeneous preparation from bovine pituitaries. A support protocol details an enzyme assay used in evaluating proteasomal activity. The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell.

References

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Citations

Jun 29, 2007·Neuro-degenerative Diseases·Julie DunysFrédéric Checler

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