Purification of the human placental alpha 2-macroglobulin receptor.

FEBS Letters
P H JensenJ Gliemann

Abstract

The alpha 2-macroglobulin receptor was solubilized from human placental membranes, purified and characterized. Affinity cross-linking of labelled ligand to intact membranes showed a receptor size compatible with 400-500 kDa. The membranes were solubilized in 3-[(3-cholamidopropyl)dimethylammonio]propane sulfonate (CHAPS) and affinity chromatography was performed using Sepharose-immobilized alpha 2-macroglobulin-methylamine with elution in buffer containing 2 mM EDTA, pH 6.0. SDS-PAGE of the resulting receptor preparation showed a predominant approx. 440 kDa band (reducing conditions) and some minor accompanying proteins of 70-90 kDa and 40 kDa. The yield was 400-800 micrograms receptor preparation per placenta. The receptor preparation immobilized on nitrocellulose bound the alpha 2-macroglobulin-trypsin complex with a dissociation constant of about 400 pM. 125I-iodinated receptor preparation bound almost quantitatively to Sepharose-immobilized alpha 2-macroglobulin-methylamine in the presence of CHAPS alone, and bound 70-80% in the presence of 0.2% SDS. The labelled proteins were separated in the presence of 0.2% SDS by gel filtration or SDS-PAGE (unboiled samples). The 440 kDa protein accounted for the major part of the bindi...Continue Reading

References

Jan 23, 1986·Biochimica Et Biophysica Acta·J Gliemann, O Davidsen
Aug 31, 1987·FEBS Letters·J Gliemann, L Sottrup-Jensen
Apr 1, 1988·European Journal of Clinical Investigation·C M PetersenJ Ingerslev
Apr 30, 1985·Biochemical and Biophysical Research Communications·S R FeldmanS V Pizzo
Mar 31, 1983·Biochimica Et Biophysica Acta·J GliemannL Sottrup-Jensen

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Citations

Aug 1, 1995·Journal of Cellular Physiology·K VettenrantaA L Schwartz
Aug 31, 2000·Cell Biology International·J T DjordjevicK K Stanley
Jan 1, 1992·Electron Microscopy Reviews·E DelainF Van Leuven
Jul 30, 1999·Developmental and Comparative Immunology·P B Armstrong, J P Quigley
Aug 30, 2008·Genes, Brain, and Behavior·P PandeyB Mittal
Sep 10, 1994·Annals of the New York Academy of Sciences·D J FitzgeraldS Leppla
Aug 1, 1993·The Journal of Clinical Investigation·I WarshawskyA L Schwartz
Jun 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Chang CaoKimberly O O'Brien
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·D K Strickland, S Ranganathan
Jun 11, 2005·Blood·Vibeke HvidbergSøren K Moestrup
Jul 21, 1995·The Journal of Biological Chemistry·A A TroussardF T Yen
Jan 5, 1999·The Journal of Biological Chemistry·L V MedvedD K Strickland
Mar 3, 2017·Biological Chemistry·Theodoros GoulasF Xavier Gomis-Rüth
Aug 14, 2019·Frontiers in Immunology·Ghee Rye LeeLeo E Otterbein
Jan 23, 1999·The Journal of Nutrition·D Beshgetoor, B Lönnerdal

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