Purification of the major outer membrane protein of Azospirillum brasilense, its affinity to plant roots, and its involvement in cell aggregation

Molecular Plant-microbe Interactions : MPMI
Saul BurdmanEdouard Jurkevitch

Abstract

The major outer membrane protein (MOMP) of the nitrogen-fixing rhizobacterium Azospirillum brasilense strain Cd was purified and isolated by gel filtration, and antiserum against this protein was obtained. A screening of the binding of outer membrane proteins (OMPs) of A. brasilense to membrane-immobilized root extracts of various plant species revealed different affinities for the MOMP, with a stronger adhesion to extracts of cereals in comparison with legumes and tomatoes. Moreover, this protein was shown to bind to roots of different cereal seedlings in an in vitro adhesion assay. Incubation of A. brasilense cells with MOMP-antiserum led to fast agglutination, indicating that the MOMP is a surface-exposed protein. Cells incubated with Fab fragments obtained from purified MOMP-antiserum immunoglobulin G exhibited significant inhibition of bacterial aggregation as compared with controls. Bacteria preincubated with Fab fragments showed weaker adhesion to corn roots in comparison to controls without Fab fragments. These findings suggest that the A. brasilense MOMP acts as an adhesin involved in root adsorption and cell aggregation of this bacterium.

References

Dec 1, 1979·Journal of Bacteriology·R E Hancock, A M Carey
Sep 1, 1995·Journal of Industrial Microbiology·P D Marsh, D J Bradshaw
Apr 16, 1998·Current Microbiology·T CastellanosY Bashan
May 20, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Nov 25, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·S BurdmanE Jurkevitch

❮ Previous
Next ❯

Citations

Jan 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Geeta SinghT S Marwaha
Nov 26, 2003·FEMS Microbiology Letters·Luis Javier Martínez-MoralesJosé Antonio Sánchez-Ahédo
May 4, 2006·Annual Review of Plant Biology·Harsh P BaisJorge M Vivanco
Jan 14, 2005·Critical Reviews in Microbiology·E SomersM Srinivasan
Jul 20, 2010·Critical Reviews in Microbiology·Swarnalee Dutta, Appa Rao Podile
Sep 16, 2008·Research in Microbiology·Mickaël BoyerFlorence Wisniewski-Dyé
Mar 13, 2013·Annals of Botany·Carole SantiClaudine Franche
Apr 20, 2018·FEMS Microbiology Reviews·Rachel M Wheatley, Philip S Poole
Jul 17, 2004·Microbiology·Emanuele G BiondiMaddalena del Gallo
Feb 28, 2020·FEMS Microbiology Letters·Víctor I Viruega-GóngoraAlberto Ramírez-Mata
Feb 5, 2021·Environmental Microbiology Reports·Hayley E KnightsPhilip S Poole
Sep 4, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Parvaze Ahmad Wani, Mohammad Saghir Khan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.