PMID: 7020758Jun 9, 1981Paper

Purification of yeast tubulin by self-assembly in vitro

Biochemistry
J V Kilmartin

Abstract

Tubulin was purified from yeast homogenate by DEAE-Sephadex column chromatography and temperature-dependent assembly. The yeast tubulin subunits comigrate with the brain alpha-tubulin subunit on one-dimensional sodium dodecyl sulfate gel electrophoresis. The in vitro yeast tubulin assembly is inhibited by the fungicide methyl N-(benzimidazol-2-yl)carbamate, the active component of benomyl, whereas in vitro brain 6S tubulin assembly is resistant. This suggests that the inhibitory effect of benomyl on yeast cell division is due to its antimicrotubule action.

References

Apr 1, 1975·The Journal of Cell Biology·P R BurtonG B Pierson
Nov 1, 1977·The Journal of Cell Biology·T WallimannH M Eppenberger
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
May 1, 1978·Journal of Bacteriology·H L Klein, B Byers
Jun 1, 1978·Journal of General Microbiology·N R Morris
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P BaumL Honig
Oct 1, 1979·FEBS Letters·L ClaytonK Gull
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Scherer, R W Davis
Feb 1, 1979·The Journal of Cell Biology·H KimJ L Rosenbaum
Sep 1, 1979·Biochemical Pharmacology·C M IrelandC I Pogson
Apr 1, 1978·Journal of Biochemistry·S Maekawa, H Sakai
Dec 18, 1978·Biochimica Et Biophysica Acta·P A Friedman, E G Platzer
Jul 23, 1976·Molecular & General Genetics : MGG·P NurseK Nasmyth
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D B Murphy, G G Borisy
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Dec 5, 1975·Journal of Molecular Biology·M W KirschnerR C Williams
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 19, 1980·Science·R B WallaceJ Abelson
Nov 1, 1980·Experimental Cell Research·A RoobolK Gull

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Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·T Stearns
Jan 1, 1982·Journal of Molecular Evolution·M LittleC F Asnes
Jun 1, 1985·Mutation Research·F K ZimmermannM A Resnick
Aug 1, 1990·Mutation Research·V W Mayer, A Aguilera
Jan 1, 1991·Pharmacology & Therapeutics·S B Hastie
Mar 1, 1983·Molecular and Biochemical Parasitology·P J DawsonK Gull
Jul 16, 1986·Biochimica Et Biophysica Acta·S A MackinlayT H MacRae
Jan 1, 1990·Electron Microscopy Reviews·E UngerW Vater
May 20, 1998·Mutation Research·M J AardemaR D Taalman
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A SuprenantW L Dentler
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T Goto, J C Wang
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L Pillus, F Solomon
Feb 1, 1985·The Journal of Cell Biology·G HuberA Matus
Jun 1, 1988·The Journal of Cell Biology·T C HuffakerD Botstein
Oct 1, 1988·The Journal of Cell Biology·C W JacobsJ R Pringle
Jun 1, 1989·The Journal of Cell Biology·S D GeorgatosG Blobel
Nov 1, 1990·The Journal of Cell Biology·M P Rout, J V Kilmartin

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