Purified herpes simplex virus thymidine kinase retroviral particles: III. Characterization of bystander killing mechanisms in transfected tumor cells

Cancer Gene Therapy
Francis J BurrowsCarol A Kruse

Abstract

An important consequence of the suicide gene therapeutic paradigm is the phenomenon of bystander cell killing, the death of adjacent tumor cells not transduced with the thymidine kinase (TK) gene from herpes simplex virus (HSV) after treatment with the antiviral drug, ganciclovir (GCV). Evidence from quantitative in vitro assays of glioma cell lines suggest that both murine and human gliomas are similar in expressing high sensitivity to the bystander effect. In five of six glial tumors examined, the presence of only 5% of HSV-TK-expressing transduced cells in the culture resulted in >90% tumor cell death/stasis after addition of GCV. Several lines of evidence support gap junction intercellular communication (GJIC) as important in the bystander effect. In vitro metabolic assays, performed with GCV in the medium, indicated that more tumor burden was reduced when culture conditions supported cell-cell contact of parental and HSV-TK-transduced cells. Additionally, a double dye transfer assay showed that cell communication through the gap junction is greatest for glioma, less for melanoma, and much less for colorectal carcinoma cell lines. In vitro metabolic assays with mixtures of TK+/TK- homologous tumor cells confirmed that gliom...Continue Reading

References

Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MesnilH Yamasaki
Jan 4, 1997·Lancet·S M FreemanA J Marrogi
Sep 5, 1997·The Journal of Biological Chemistry·M Y KanemitsuW Eckhart
May 16, 1998·The Journal of Biological Chemistry·B J Warn-CramerA F Lau
Feb 23, 1999·Advances in Experimental Medicine and Biology·R RameshS M Freeman
Jun 11, 1999·Biochemical and Biophysical Research Communications·S BaiL He
Sep 25, 1999·International Journal of Cancer. Journal International Du Cancer·S KuriyamaK Ikenaka

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Citations

May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·D V KryskoK D'Herde
Mar 14, 2009·Human Gene Therapy·Jordi Martinez-QuintanillaRamon Alemany
Aug 28, 2003·Acta Physiologica Scandinavica·J C SáezM V L Bennett
Jan 27, 2005·Biochemical and Biophysical Research Communications·Martin UhlUlrich Herrlinger
Dec 2, 2004·Brain Research. Brain Research Reviews·Jorge E ContrerasJuan C Sáez
Apr 23, 2014·International Journal of Cancer. Journal International Du Cancer·Solveig SirnesEdward Leithe
Jul 26, 2003·Expert Opinion on Biological Therapy·D J KerrF Maruta
Mar 18, 2015·Current Treatment Options in Oncology·Neal DharmadhikariHoward L Kaufman
Apr 6, 2007·Pediatric Neurosurgery·Pablo F RecinosGeorge I Jallo
Jul 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mini Thomas, Alexander M Klibanov
Aug 2, 2003·Nature Reviews. Cancer·David Kerr
Aug 27, 2018·Current Oncology Reports·Claire-Audrey Y BayanYvonne M Saenger
Nov 6, 2009·Molecular Cancer Therapeutics·Jordi Martinez-QuintanillaRamon Alemany
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia

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