Purinergic agonist ATP is a comitogen in thyroid FRTL-5 cells

Journal of Cellular Physiology
K TörnquistB Dugué

Abstract

Several growth factors may stimulate proliferation of thyroid cells. This effect has, in part, been dependent on calcium entry. In the present study using FRTL-5 cells, we show that in addition to its effect on calcium fluxes, ATP acts as a comitogen in these cells. In medium containing 5% serum, but no TSH, ATP stimulated the incorporation of 3H-thymidine in a dose- and time-dependent manner in the cells. At least a 24-h incubation with ATP was necessary to observe the enhanced (30-50%) incorporation of 3H-thymidine and an increased (30%) cell number. The effect of ATP was dependent on insulin in the incubation medium. Furthermore, ATP enhanced the TSH-mediated incorporation of 3H-thymidine. The effect of ATP was apparently mediated via a G-protein dependent mechanism, as no stimulation of thymidine incorporation was observed in cells treated with pertussis toxin. The effect of ATP was not dependent on the activation of protein kinase C (PKC), as ATP was effective in cells with downregulated PKC. ATP rapidly phosphorylated mitogen activated protein (MAP) kinase in FRTL-5 cells. In addition, ATP stimulated the expression of a 62 kDa c-fos dependent protein in a dose- and time-dependent manner. Our results thus suggest that extr...Continue Reading

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Citations

Jun 28, 2000·Journal of the Autonomic Nervous System·F Di Virgilio
Aug 26, 1998·Thyroid : Official Journal of the American Thyroid Association·V TasevskiA Simpson
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John T SwarthoutStephen C J Efcoat
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Mar 27, 2001·Journal of Cellular Physiology·E EkokoskiK Törnquist
Jan 20, 2010·Biochimica Et Biophysica Acta·Stephan LorenzKnut Krohn
Nov 26, 1998·Japanese Journal of Pharmacology·M P Abbracchio, G Burnstock
Apr 12, 2014·Pflügers Archiv : European journal of physiology·Kid TörnquistTero Viitanen
Oct 25, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefania MariggiòDaniela Corda
Apr 3, 1998·Journal of Cellular Physiology·K TörnquistE Ekokoski
Dec 7, 2007·Journal of Cell Science·Sonja BuvinicAlfonso González
May 1, 2021·Cancers·Muhammad Yasir AsgharKid Törnquist

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